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KMID : 0613820060160050859
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Journal of Life Science
2006 Volume.16 No. 5 p.859 ~ p.865
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Screening of Endogenous Maize (Zea mays) Substances Enhancing Auxin-induced Inward Curvature in Coleoptilar Slits
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Park Woong-June
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Abstract
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When thin slits (e.g., 1 mm x 10 mm) of maize (Zea mays) coleoptiles were floated on a buffer, they spontaneously curved outward because of unbalanced tissue tension between inner and outer faces. Exogenously applied auxin induced inward curvature of the thin strip of the maize coleoptile in a dose-dependent manner. This bioassay system was used to screen endogenous substances that work together with auxin. In methanol extract of maize coleoptiles including the leaves inside, Active fractions that promote the auxin-induced inward curvature of maize coleoptile slices were found. The curvature-enhancing activity of the extract was not related to energy supply. The active substances were adsorbed to C₁? cartridges even at pH 10 and eluted in two fractions by 50% and 80% methanol. These substances were named as Curvature-Enhancing Factor-1 (CEF-1) and Curvature-Enhancing Factor-2 (CEF-2), respectively. The CEF-2 was resolved on a reversed phase C₁? column by HPLC.
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KEYWORD
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Auxin-induced curvature, coleoptile, endogenous regulator, Zea mays
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